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NIH3D

Modified Hiraoka TEM Grid Staining Apparatus

Created by
pseifert-meei
Created:
7/17/17
Submitted:
5/3/24
Published:
5/3/24

Select an image below to view

3DPX-007685

Licensing:

CC-BY-NC-SA
126
2
Version 2.01

Category

Hardware & Devices
Devices and Hardware
Description

The commercially unavailable Hiraoka transmission electron microscopy (TEM) grid staining apparatus (1972) allows multiple grids to be batch stained together on a support plate.  The 3D printed modified Hiraoka TEM grid staining apparatus is designed to fit a Leica AC20 grid plate holder and trays lined with ParafilmR, comprised of four pieces;

           (1)    Modified Hiraoka staining tray

           (2)    Modified Hiraoka plate holder

           (3)    ParafilmR well mold

           (4)    Staining tray space insert

 

Please contact me for further details and information on TEM grid staining procedure using this apparatus for conventional biological TEM applications.  Refer to the following article for more details;

Seifert, P. (2017). Modified Hiraoka TEM grid staining apparatus and technique using 3D printed materials and gadolinium triacetate tetrahydrate, a nonradioactive uranyl acetate substitute. Journal of Histotechnology40(4), 130–135. https://doi.org/10.1080/01478885.2017.1361117


Apparatus for Automated TEM Grid staining using the HistoEnder (Ender-3 Printer)

by pseifert-meei


Apparatus for Automated TEM Grid staining using the HistoEnder (Ender-3 Printer)