Automated TEM Grid staining using the HistoEnder (Ender-3 Printer): 

TH_Hiraoka tray matrix1.stl

A 4 x 4= 16 fused modified Hiraoka TEM Grid Staining Tray matrix piece for staining TEM grids.  Each tray can hold up to 4 mL of heavy metal stains (e.g. aqueous uranyl acetate, Lead citrate stain) and distilled water rinses.  It is recommended to print in a chemical resistant material (e.g. PETG, ABS, or polypropylene) and spray a water resistant sealant over the tray wells.  Parafilm lining using the modified Hiraoka kit apparatus should be used for maintaining a clean surface and aid in disposal. 

TH_GridPlate Holder_A and TH_GridPlate Holder_B

Interfaces with HistoEnder Headpiece and acts as a Holder for the Leica EM AC20 grid support plate (Leica Microsystems, U.S.A. 38×40mm, cat# 16707299). Parafilm strips may be used to retain the plate and prevent chemical contamination of the holder. 

TH_GridPlate Holder_A.stl  

Has a T-hinge to attach to the HistoEnder Headpiece. Note: Loaded with steel washers for magnetism and weight, then glued to  piece TH_GridPlate Holder_B.stl 

TH_GridPlate Holder_B.stl: Holds the Leica EM AC20 grid support plate. 

Note: TH_GridPlate Holder_B.stl: needs to be glued to piece TH_GridPlate Holder_A.stl 

Detailed in publication:

Seifert, P. (2024). Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining. Journal of Histotechnology, 1–5.

https://doi.org/10.1080/01478885.2024.2403872