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National Institute of Allergy and Infectious Disease
NIH 3D
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NIH3D

IgG Rabbit Polyclonal Antibody Conjugated to Split Beta-Galactosidase Alpha Subunit for Proximity Based Ligation Detection of Analytes

Generated by NIH 3D workflows using data provided by
Rahul Madhu
Created:
3/21/26
Submitted:
3/21/26
Published:
3/21/26
ribbon , colored by secondary structure, not intended for 3D printing
IgG_AlphaFinal-ribbon-secondary-vis_NIH3DRibbon representation colored by secondary structure (a-helix: blue, B-sheet: red, coil: yellow). Not intended for 3D Printing

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3DPX-023394

Licensing:

CC-BY-ND
How to cite this NIH 3D entry
21
4
Version 1

Category

Biomacromolecules
Biomacromolecules
Description

Description: This 3D model represents a diagnostic Immunoglobulin G (IgG) antibody conjugated to the alpha subunit of beta-galactosidase, specifically the N-terminal fragment spanning residues 1-77 (the Enzyme Donor). In the ESSENCE nanoplatform, this alpha-donor fragment is an inactive polypeptide that restores full enzymatic activity only upon binding with its larger complementary partner, the omega-acceptor (the M15 deletion mutant).

Technical Significance:

  • Complementation: The 1-77 fragment acts as the essential key that restores the structural integrity of the enzyme active site and stabilizes the tetrameric state.

  • Readout: Reconstitution of the functional enzyme triggers the hydrolysis of colorimetric substrates, enabling the wash-free detection of skin cancer biomarkers (IL-1alpha or CTSK).

  • Prototyping: This model allows for the precise spatial mapping of the enzyme donor within the vertical channels of the diagnostic patch.

Structural Note: This model is rendered as a high-fidelity surface mesh to illustrate the relative scale between the large IgG scaffold (150 kDa) and the smaller alpha-peptide donor (8.5 kDa).

Source Data Details