Save costs by printing your own vacuum manifold instead of purchasing an expensive one.

This 3D-printed vacuum manifold is designed to fit the 96-well filter plate used in BioLegend LEGENDplex bead-based immunoassays.

Tips for achieving an airtight print:

• Use PETG instead of PLA for better durability and performance.
• Print at a high temperature (I used 255°C) to ensure strong layer adhesion.
• Keep your filament dry to avoid bubbling and potential defects/leaks
• Set a high resolution for the print (I used 0.12 mm).
• Opt for random seam positioning to prevent leakage through the seams
• Increase the number of perimeters (I used 4) to reduce the potential for leaks through the walls.
• Print with more top layers (I used 6) to improve surface integrity.
• Reduce print speed by about 25% for better precision.
• (Optional) Apply vacuum grease where the manifold meets the 96-well plate to improve the seal.