The Inclined Salamander Positioning Device (ISPD) is a 3-D printed device designed to assist with the collection of sperm from axolotls and has potential uses for other salamander species. This device could also be applied to veterinary procedures involving salamanders. The device can be scaled to accommodate a range of body sizes.

The animals pictured are anesthetized in compliance with IACUC policies.

Please click the 'DOCUMENTATION' tab to download the standard operating procedure (SOP) used at the center for axolotls.

To learn more about axolotl handling, sperm collection, and cryopreservation, please view our complete publication in the PDF attached or the following link. Coxe, N.; Liu, Y.; Arregui, L.; Upton, R.; Bodenstein, S.; Voss, S.R.; Gutierrez-Wing, M.T.; Tiersch, T.R. Establishment of a Practical Sperm Cryopreservation Pathway for the Axolotl (Ambystoma mexicanum): A Community-Level Approach to Germplasm Repository Development. Animals 2024, 14, 206. https://doi.org/10.3390/ani14020206

Developed by the Aquatic Germplasm and Genetic Resources Center (AGGRC). Originally designed by Nikolas Carey, Madison Adams, and Nicholas Coxe.

Please visit our website (AGGRC.com) to learn more about our work.

All Fusion 360 files can be found on our GitHub through the following link:

https://github.com/aggrc/Inclined-Salamander-Positioning-Device-ISPD-or-Axo-Lounge