We designed a 3D-printed X-ray chamber, which allows for in-situ exchange of buffer and in-situ optical transmission spectroscopy. The chamber is made of cyclic olefin copolymers (COC) including COC X-ray windows providing ultra-low SAXS background. The design integrates a membrane insert for in-situ dialysis of the 100ul sample volume against a reservoir, enabling measurements of the same sample under multiple conditions using an in-house X-ray setup equipped with a 17.4 keV molybdenum source.