A pipet guide designed similarly to the Seahorse transfer system made by Agilent. While the Agilent system is produced for a single column with 8 positions for pipet tips, the custom guide is designed to accommodate 96 wells at once (both versions included here). Using the guide, cultured cancer spheroids can be easily transferred from hanging drops and placed in the centers of wells in the spheroid assay plates already containing assay media. The spheroids are collected from the hanging drops using a multichannel pipette and tips are then placed in the guide and the spheroids allowed to drop out of the tips by gravity to the well surface below. While other tips stay in the guide, more tips can be added. The way it’s been used presently is in a XF96 spheroid microplate for dynamic measurement on the Agilent Seahorse Cell Analyzer. I foresee that this could be used in many applications where the same sort of high-throughput and precise placement is needed, mainly 3D culture of cells. The design may need to be changed to accommodate different plates than the Seahorse plates, however.