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NIH3D

Hepatitis B Relaxed Circular DNA, condensed (PathogenAR)

Created by
kbrowne
Created:
7/23/21
Submitted:
8/16/23
Published:
8/16/23

Select an image below to view

3DPX-015990

Licensing:

CC-BY
192
9
Version 3

Category

Biomacromolecules
Biomacromolecules

Collection(s)

Description

This model approximates RC DNA when folded inside the capsid.

The RC DNA was built by snapping a curve path to the interior of the capsid surface in Maya, then shrinking it to provide some extra space.  ~3200bp of DNA was then created along the path using Molecular Maya's dsDNA Rigging Kit (https://clarafi.com/training/intro-mmaya-dsdna-kit).  RT from HIV was used as a very general approximation of P Protein as a structure was not available. PDB 1T03</color> was fitted to the RC DNA and bases were removed from the strand as appropriate.

An illustration of the life cycle from PathogenAR is included, with the step associated with this model highlighted.

This model was used in the PathogenAR Hepatitis B: The Hepatitis B Life Cycle Module in PathogenAR (https://www.niaid.nih.gov/research/pathogenar-mobile-app)

ZBrush rendering of condensed DNA model
hepB life cycle diagram highlighting where this model is relevant