Fluorescence is a form of photoluminescence. This natural phenomenon occurs when a material absorbs light and subsequently emits photons with longer wavelengths. The discovery of fluorescence and the utilization of wavelength filters has aided the ability of basic and clinical scientists to visualize the structure and function of cells and tissues. Intrinsic autofluorescence or manipulation of florescence via fluorophore conjugated proteins, allows for the visual isolation of a cell or tissue based on its unique excitation and emission signature. The addition of one or more wavelength filters to the imaging pathway allows for visualization of specific regions of the emission spectrum that are associated with the unique fluorescence signatures of the cells or tissues of interest.

 

The filter adapter consists of two components: 1) a filter ring with a 23mm microscope mount on one end and a filter receptacle (accepts one or two 6.3mm x 25.4mm (HxD) filters*) on the other end, and 2) a camera ring with a filter ring receptacle on one end and a 23mm camera receptacle on the other end.

 

*https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=918