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National Institute of Allergy and Infectious Disease
NIH 3D
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NIH3D

Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2

Generated by NIH 3D workflows using data provided by
asawant
Created:
4/17/19
Submitted:
3/6/23
Published:
3/6/23
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3DPX-010964

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CC-BY
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168
41
Version 2

Category

Biomacromolecules
Biomacromolecules
Description

The CRISPR-associated protein Cas9 is an RNA-guided endonuclease that cleaves double-stranded DNA bearing sequences complementary to a 20-nucleotide segment in the guide RNA. Cas9 has emerged as a versatile molecular tool for genome editing and gene expression control. RNA-guided DNA recognition and cleavage strictly require the presence of a protospacer adjacent motif (PAM) in the target DNA. Streptococcus pyogenes Cas9 in complex with a single-molecule guide RNA and a target DNA containing a canonical 5'-NGG-3' PAM. 

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