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National Institute of Allergy and Infectious Disease
NIH 3D
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NIH3D

Modified Hiraoka TEM Grid Staining Apparatus

Created by
pseifert-meei
Created:
7/17/17
Submitted:
10/5/26
Published:
10/5/26
.stl file for modified Hiraoka support plate
hiraoka_support_plate_holder_final.stl file for modified Hiraoka support plate.

Select an image below to view

.stl file for modified Hiraoka support plate.stl file for modified Hiraoka staining tray.stl file for modified Hiraoka well mold. For molding Parafilm into the modified Hiraoka staining tray well.stl file for modified Hiraoka staining tray insert

Learn more about NIH 3D workflow outputs

3DPX-007685

NIH 3D DOI:

https://doi.org/10.60705/3dpx/7685.3

Licensing:

CC-BY-NC-SA
How to cite this NIH 3D entry
414
33
Version 3

Category

Hardware & Devices
Devices and Hardware
Description

The commercially unavailable Hiraoka transmission electron microscopy (TEM) grid staining apparatus (1972) allows multiple grids to be batch stained together on a support plate.  The 3D printed modified Hiraoka TEM grid staining apparatus is designed to fit a Leica AC20 grid plate holder and trays lined with ParafilmR, comprised of four pieces;

           (1)    Modified Hiraoka staining tray

           (2)    Modified Hiraoka plate holder

           (3)    ParafilmR well mold

           (4)    Staining tray space insert

 

Please contact me for further details and information on TEM grid staining procedure using this apparatus for conventional biological TEM applications.  Refer to the following article for more details;

Seifert, P. (2017). Modified Hiraoka TEM grid staining apparatus and technique using 3D printed materials and gadolinium triacetate tetrahydrate, a nonradioactive uranyl acetate substitute. Journal of Histotechnology, 40(4), 130–135. https://doi.org/10.1080/01478885.2017.1361117


Source Data Details
Attribution Instructions
3D Printing Instructions
Modified Hiraoka plate holder printed in ABS
Modified Hiraoka staining tray (ABS) and insert (ABS) lined with Parafilm and liquid
Modified Hiraoka- Parafilm well mold printed in ABS